A bull’s fertility is determined by the ability of the sperm to fertilize the ovum to create a new individual (zygote). The bull fertility level is very influential on the reproduction efficiency, which is essential for effective and efficient breeding. The application of artificial insemination (AI) as a reproduction technology takes advantage of frozen semen from a first-rate bull species in which the sperm could still be alive during the process of cryopreservation and impact the success of insemination. The quality of frozen semen used for the insemination is considered to be significantly associated with the percentage of conception.