The ejaculate volume, color, consistency, sperm concentration, and initial motility in pure and crossbred Jersey bulls did not differ. Mass activity, live sperm percentage, and acrosome integrity were significantly higher in pure Jersey bulls as compared to crossbred Jersey bulls. No statistical difference was observed in abnormal sperm; HOS reacted spermatozoa and DNA integrity percentage between breeds. Significant decrease in the motility, live sperm percentage, HOS reacted spermatozoa, acrosome integrity and significant increase in abnormal count from neat to pre-freeze and from pre-freeze to post-freeze semen of pure and crossbred Jersey bulls was due to the deleterious effect of cooling-freezing and thawing procedures. The semen quality of pure Jersey bull semen was better than the crossbred bull. It may be concluded that the estimation of live sperm percentage and acrosome integrity is useful in the assessment of semen quality in bulls.